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walter: An altered white gene for use as a repair template:

The six exons of the white gene are shown as red cylinders. The whole gene is inserted between P element ends to allow placing it at various places in the genome. A set of marker sites (red dots) were engineered into walter to allow detection of conversion. Each marker alters a restriction recognition site but does not change the amino acid sequence encoded.

References:

• Nassif, N. A., J. Penney, S. Pal, W. R. Engels and G. B. Gloor. 1994. Efficient copying of nonhomologous sequences from ectopic sites via P element-induced gap repair. Mol. Cell. Biol. 14:1613-1625.
• Gloor, G. B., N. A. Nassif, D. M. Johnson-Schlitz, C. R. Preston and W. R. Engels. 1991. Targeted gene replacement in Drosophila via P element-induced gap repair. Science 253:1110-1117.
  

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